- 中文名称
Fetoprotein, Alpha, Human, BioAssay ELISA Kit (AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP)
- 英文名字
- Fetoprotein, Alpha, Human, BioAssay ELISA Kit (AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP)
- 供应商
- USBiological
- 产品货号
- F4100-11M
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Alpha-fetoprotein (AFP) is a glycoprotein of 590aa containing 3.4% carbohydrate content with a molecular weight of 61-75kD. AFP is normally produced in the developing embryo and fetus by the fetal yolk sac, the fetal gastrointestinal tract, and eventually by the fetal liver. In humans, AFP levels decrease gradually after birth, reaching adult levels by 8 to 12 months. Normal adult AFP levels are low and AFP has no known function in normal adults. The biologic role of AFP has not been defined yet. Because of its biochemical similarity to albumin, it has been postulated that AFP could be a carrier protein. It may have an immunoregulatory function during pregnancy. Increased serum levels are found in some tumors, such as hepatocellular carcinoma (HCC), hepatoblastoma, and germ cell tumors. Although total AFP is a useful serological marker for diagnosis of HCC, the false-negative or positive rate with AFP level is very high. AFP-L3, an isoform of AFP which binds Lens culinaris agglutinin, can be particularly useful in early identification of aggressive tumors associated with HCC. AFP mRNA, the circulating genetic markers, also has been used in monitoring distal metastasis or postoperative recurrence of HCC.Intended Use:Human Alpha Fetoprotein (human AFP) ELISA kit is to be used for the in vitro quantitative determination of human AFP in human serum, human plasma, cell lysate and buffered solution. The assay will recognize both native and recombinant human AFP. This kit has been configured for research use only and is not to be used in diagnostic procedures.Sensitivity: The minimal detectable dose of human AFP was calculated to be 1.82ng/ml, by subtracting two standard deviations from the mean of 12 zero standard replicates (ELISA buffer, S0) and intersecting this value with the standard curve obtained in the same calculation.Specificity:The following substances were tested and found to have no cross-reactivity: human serum albumin, human prostate specific antigen(PSA), human carcinoembryonic antigen(CEA), human neuron specific enolase(NSE), human Vitamin D binding protein(VDBP), HemoglobinTest Principle:The design of this assay is based on a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to human AFP. Samples are pippetted into these wells. Nonbound AFP and other components of the sample should be removed by washing, then biotin-conjugated monoclonal antibody specific to AFP added. In order to quantitatively determine the amount of AFP present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) should be added to each microplate well. The final step, a TMB-substrate solution added to each well. Finally, a sulfuric acid solution is added and the resulting yellow colored product is measured at 450nm. Since the increases in absorbency is directly proportional to the amount of captured AFP.Kit Components:Microplate: 1x96 wellsIncubation buffer: 1x30mlWashing buffer, (20x): 2x25mlStandard protein: 1x1vialStandard/sample dilution buffer: 1x25mlSecondary antibody: 1x1vialAV-HRP, (100X): 1x150ulSecondary antibody/AV-HRP dilution buffer: 1x25mlSubstrate (TMB): 1x15mlStop solution: 1x15mlStorage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Fetoprotein, Alpha, Human, BioAssay ELISA Kit (AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Fetoprotein, Alpha, Human, BioAssay ELISA Kit (AFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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