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细胞分析试剂盒
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中文名称

Ferritin, Human, BioAssay ELISA Kit

英文名字
Ferritin, Human, BioAssay ELISA Kit
供应商
USBiological
产品货号
F4015-07H
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
One of the most prevalent disorders of man is the dietary deficiency of iron and the resulting anemia. Therefore, the assays of iron, total iron binding capacity and other assessments of iron compounds in the body are clinically significant. Iron-storage compounds in the body include hemoglobin, hemosiderin, myoglobulin and the cytochromes. In most tissues, ferritiin is a major iron-storage protein. Human ferritin has a molecular weight of approximately 450,000D, and consists of a protein shell around an iron core; each molecule of ferritin may contain as many as 4000 iron atoms. Under normal conditions, this may represent 25% of the total iron found in the body. In addition, ferritin can be found in several isomers. High concentrations of ferritin are found in the cytoplasm of the reticuloendothelial system, the liver, spleen and bone marrow. Methods previously used to measure iron in such tissues are invasive, cause patient trauma and lack adequate sensitivity. The measurement of ferritin in serum is useful in determining changes in body iron storage, and is non-invasive with relatively little patient discomfort. Serum ferritin levels can be measured routinely and are particularly useful in the early detection of irondeficiency anemia in apparently healthy people. Serum ferritin measurements are also clinically significant in the monitoring of the iron status of pregnant women, blood donors, and renal dialysis patients. High ferritin levels may indicate iron overload without apparent liver damage, as may be noted in the early stages of idiopathic hemochromatosis. Ferritin levels in serum have also been used to evaluate clinical conditions not related to iron storage, including inflammation, chronic liver disease, and malignancy.Sample Type: SerumIntended Use: For the quantitative determination of Human Ferritin concentration in human serum.Test Principle: The Ferritin Quantitative Test is based on a solid phase enzymelinked immunosorbent assay (ELISA). The assay system utilizes one rabbit anti-ferritin antibody for solid phase (microtiter wells) immobilization and a mouse monoclonal anti-ferritin antibody in theantibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45-minute incubation at room temperature, the wells are washed with water to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated at room temperature for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, and the color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of ferritin is directly proportional to the color intensity of the test sample.Kit Components:Antibody coated microtiter plate with 1x96 wells.Enzyme Conjugate Reagent, 1x13ml.Standard, 0ng/ml 1x1vialStandard, 15ng/ml, 1x1vial Standard, 80ng/ml, 1x1vialStandard, 250ng/ml, 1x1vialStandard, 500n/ml, 1x1vialStandard, 1000ng/ml, 1x1vialTMB Reagent (One-Step), 1x11ml.Stop Solution (1N HCl), 1x11ml.Storage and Stability:Store all components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对Ferritin, Human, BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Ferritin, Human, BioAssay ELISA Kit产品后将其置于-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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