Description:Alkaline phosphatase is commonly used in Immunohistrochemistry and in situ hybridization. FastRed/Napthol AS-Mx is the most commonly used substrate chromogen of alkaline phosphatase. The end product is a pink color precipitate. Components in this kit are prepared with pure chemicals according to the proprietary technology. One Kit is consisted of two components: 5ml of 20 x Solution A and 5ml of 20 x Solution B. It is enough for staining 500 standard tissue sections. The end product is a pink color precipitate. This product is not compatible with organic solvents.Quality Control:The solution of each lot are tested by immunohistochemistry, and the results are consistent lot to lot.Protocol:Both Solution A and B are 20 x concentrated solution. For preparation of 1ml substrate working solution, add 50ul of Solution A to 900ul of distilled water, mix well. Then add 50ul of Solution B to the mixture, mix well. This is ready to use working solution. Add the working solution to cover the tissue specimen. Incubate at room temperature until enough color developed. The end product is pink color precipitate.
