- 中文名称
FAK, phosphorylated (Tyr397), Human (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) BioAssay ELISA Kit
- 英文名字
- FAK, phosphorylated (Tyr397), Human (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- F0019-55H
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Focal Adhesion Kinase (FAK), also known as pp125FAK and FADK 1 (EC,2.7.1.112) is a non-receptor protein-tyrosine kinase that localizes to focal adhesions. FAK appears to be ubiquitously expressed among all mammalian tissues, with highest expression levels observed in brain tissue. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK’s amino-terminal, which bears homology with the band 4.1 family of proteins, plays a role in mediating interaction with the cell membrane, the cytoskeleton, and integrin proteins. FAK’s carboxyl-terminus, which contains the focal adhesion targeting (FAT) domain, mediates interaction with focal adhesion associated proteins, including talin and paxillin. FAK is activated by phosphorylation events in response to several stimuli. These stimuli include integrin clustering induced by cell adhesion or antibody cross-linking, G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or by LDL receptor occupancy.In response to integrin engagement, FAK is autophosphorylated at tyrosine residue 397. This autophosphorylation event creates high affinity binding sites for the SH2 domains of several important signaling proteins, including Src family members (c-Src and Fyn, which are recruited to sites of adhesion and subsequently activated), phosphatidylinositol-3-kinase, phospholipase-Cγ and Shc. In addition to tyrosine 397, five other tyrosine residues are phosphorylated in response, including tyrosine residues 407, 576, 577, 861, and 925.Studies performed in vitro suggest that c-Src is the activity that catalyzes the phosphorylation of these residues. Phosphorylation of tyrosine 925, an event which creates a binding site for the Grb2 SH2 domain, contributes to integrin-sitmulated activation of the -ERK2/mitogen-activated protein kinase pathway. FAK promoted phosphorylation of these substrates and subsequent recruitment of Crk are critical downstream signaling events of FAK. FAK is under investigation in several areas of research, including cell adhesion and migration studies, studies on migration in response to growth factors, differentiation, cell cycle, apoptosis, and cancer studies. FAK is overexpressed in breast, colon and thyroid cancers, possibly contributing to some features of the malignant phenotype (i.e., increased migration and invasion of surrounding stroma). The role of FAK in contributing to cells’ resistance to apoptosis is also under investigation, as over-expression of FAK in Madin-Darby canine kidney cells has been found to render these cells resistant to apoptosis following loss of adherence.FAK [pY397] ELISA is designed to detect and quantify the level of FAK protein phosphorylated at tyrosine residue 397. This assay is intended for the detection of FAK [pY397] from lysates of human and mouse cells. Also available is a FAK (Total) ELISA kit, which quantifies FAK independently of phosphorylation status and allows normalization of phosphorylated FAK to total FAK.
- 产品特点
- 针对FAK, phosphorylated (Tyr397), Human (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到FAK, phosphorylated (Tyr397), Human (Focal Adhesion Associated Protein Tyrosine Kinase, BC3) BioAssay ELISA Kit产品后将其置于4°C (-20°C Glycerol)保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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