Acyl-CoA Thioesterase 8（ACOT8) is a group of enzymes which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A（CoASH)， granting the potential to regulate intracellular levels of acyl-CoAs， free fatty acids and CoASH. ACOT8 mediate Nef-induced down-regulation of CD4. ACOT8 contends with BAAT（Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate（such as chenodeoxycholoyl-CoA). ACOT8 prefers medium-length fatty acyl-CoAs.
