Polymerase III Polypeptide F（POLR3F) is a member of the eukaryotic RPC34/RPC39 RNA polymerase subunit family. POLR3F is DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the 4 ribonucleoside triphosphates as substrates. POLR3F is one of many subunits forming eukaryotic RNA polymerase III（RNA Pol III)， which transcribes 5S ribosomal RNA and tRNA genes. POLR3F binds both TFIIIB90 and TBP， 2 subunits of RNA polymerase III transcription initiation factor IIIB（TFIIIB).
