CDC34 is important in the control of cell cycle and DNA replication. Cdc34 in association with different E3 complexes， including SCF， has been shown to target many different substrates for ubiquitination and degradation during cell division， signal transduction， and development. Cdc34 substrates that have been characterized include I?B， B-Myb， Wee1， MyoD， ICERII?， ATF5， p27Xic1， and p27Kip1. Additionally， substrates such as ?-catenin， p21Cip1， E2F， cyclin E， and cyclin D are putative substrates of Cdc34 by virtue of their SCF requirement for proteolysis. Cdc34 has been demonstrated to self-associate through a domain in the C-terminus， and is phosphorylated and ubiquitinylated in vivo. This protein is useful for in vitro ubiquitinylation reactions.
序列
MHHHHHHAMGILMARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEG
DLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPN
IYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPN
TFSPANVDASVMYRKWKESKGKDREYTDIIRKQVGTKVDAERDGVKVPT
TLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGT
EES.
