Phospholipase A2（PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid（AA)， a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids， which represent another class of lipid mediators.
The secretory PLA2（sPLA2) family， in which 10 isozymes have been identified， consists of low molecular weight， Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes， such as modification of eicosanoid generation， inflammation， and host defense.
This enzyme has been proposed to hydrolyze phosphatidylcholine（PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall， thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.
In mice， sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large: small ratio of surfactant aggregates in rats.
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来源宿主
大肠杆菌中重组表达
溶解建议
提供的人分泌磷脂酶A2-IID（PLA2G2D）产品为过滤灭菌的冻干粉末；该冻干粉末是从含0.5mg/ml人分泌磷脂酶A2-IID（PLA2G2D）的0.05M醋酸盐缓冲液（pH 4）中过滤灭菌并冻干产生的.我们建议加0.2 ml 0.1M 醋酸盐缓冲液（pH 4）以配制工作储存液，使粉末完全溶解.如要使pH值更大，我们建议通过相关缓冲液进一步稀释到10ug/ml.过高的浓度会影响PLA2G2D的溶解性.
