This assay has high sensitivity and excellent specificity for detection of Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B). No significant cross-reactivity or interference between Anti-Myosin Heavy Chain 7B, Cardiac Muscle Beta Antibody (Anti-MYH7B) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 1 (GAL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 1 (GAL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 1 (GAL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 1 (GAL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Competitive Inhibition
保存建议
Biomatik品牌厂家建议该人抗肌球蛋白重链7B，心肌Beta抗体(Anti-MYH7B)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
