This assay has high sensitivity and excellent specificity for detection of Deoxycytidine Triphosphate Pyrophosphatase 1 (DCTPP1). No significant cross-reactivity or interference between Deoxycytidine Triphosphate Pyrophosphatase 1 (DCTPP1) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Preferentially Expressed Antigen In Melanoma (PRAME). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Preferentially Expressed Antigen In Melanoma (PRAME). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Preferentially Expressed Antigen In Melanoma (PRAME), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Preferentially Expressed Antigen In Melanoma (PRAME) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人脱氧胞苷三磷酸焦磷酸酶1(DCTPP1)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
