This assay has high sensitivity and excellent specificity for detection of Ras Related C3 Botulinum Toxin Substrate 1 (Rac1). No significant cross-reactivity or interference between Ras Related C3 Botulinum Toxin Substrate 1 (Rac1) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCNA Associated Factor 15kDa (PAF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PCNA Associated Factor 15kDa (PAF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCNA Associated Factor 15kDa (PAF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PCNA Associated Factor 15kDa (PAF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该小鼠Ras相关C3肉毒杆菌毒素底物1(Rac1)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
