This assay has high sensitivity and excellent specificity for detection of Wide-range Fibroblast Growth Factor 15 (FGF15). No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 15 (FGF15) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hexa Histidine (H6) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hexa Histidine (H6) and unlabeled Hexa Histidine (H6) (Standards or samples) with the pre-coated antibody specific to Hexa Histidine (H6). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hexa Histidine (H6) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hexa Histidine (H6) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠成纤维细胞生长因子15(FGF15)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
