This assay has high sensitivity and excellent specificity for detection of Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D). No significant cross-reactivity or interference between Inositol Polyphosphate-5-Phosphatase 145kDa (INPP5D) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人肌醇多磷酸-5-磷酸酶145kDa(INPP5D)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
