This assay has high sensitivity and excellent specificity for detection of Leucine Rich PPR Motif Containing Protein (LRPPRC). No significant cross-reactivity or interference between Leucine Rich PPR Motif Containing Protein (LRPPRC) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 27 (TMEM27). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 27 (TMEM27). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 27 (TMEM27), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane Protein 27 (TMEM27) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人富含亮氨酸的PPR基序含蛋白(LRPPRC)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
