This assay has high sensitivity and excellent specificity for detection of 5'-Nucleotidase, Cytosolic II (NT5C2). No significant cross-reactivity or interference between 5'-Nucleotidase, Cytosolic II (NT5C2) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frizzled Homolog 6 (FZD6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frizzled Homolog 6 (FZD6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frizzled Homolog 6 (FZD6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Frizzled Homolog 6 (FZD6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠5'-核苷酸酶，胞质II(NT5C2)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
