This assay has high sensitivity and excellent specificity for detection of Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP). No significant cross-reactivity or interference between Lectin Galactoside Binding, Soluble 3 Binding Protein (LGALS3BP) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 5 (ITGb5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 5 (ITGb5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 5 (ITGb5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 5 (ITGb5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠凝集素半乳糖苷结合，可溶性3结合蛋白(LGALS3BP)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
