This assay has high sensitivity and excellent specificity for detection of Troponin T Type 3, Fast Skeletal (TNNT3). No significant cross-reactivity or interference between Troponin T Type 3, Fast Skeletal (TNNT3) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Preprovasopressin (VP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Preprovasopressin (VP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Preprovasopressin (VP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Preprovasopressin (VP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人肌钙蛋白T3型，快速骨骼(TNNT3)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
