This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 6 (STAT6). No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 6 (STAT6) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-Motif Ligand 2 (XCL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-Motif Ligand 2 (XCL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-Motif Ligand 2 (XCL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-Motif Ligand 2 (XCL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠信号转导和转录激活因子6(STAT6)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
