This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A). No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, cAMP Dependent Catalytic Alpha (PRKACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠信号素3A(SEMA3A)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
