This assay has high sensitivity and excellent specificity for detection of Octamer Binding Transcription Factor 4 (OCT4). No significant cross-reactivity or interference between Octamer Binding Transcription Factor 4 (OCT4) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen II C-Terminal Propeptide (PIICP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen II C-Terminal Propeptide (PIICP) and unlabeled Procollagen II C-Terminal Propeptide (PIICP) (Standards or samples) with the pre-coated antibody specific to Procollagen II C-Terminal Propeptide (PIICP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该小鼠八聚体结合转录因子4(OCT4)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.