This assay has high sensitivity and excellent specificity for detection of Leptin (LEP). No significant cross-reactivity or interference between Leptin (LEP) and analogues was observed.
产品描述
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Indole 3 Acetic Acid (IAA). A competitive inhibition reaction is launched between biotin labeled Indole 3 Acetic Acid (IAA) and unlabeled Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Indole 3 Acetic Acid (IAA) level in the sample or standard.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠瘦素(LEP)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
