This assay has high sensitivity and excellent specificity for detection of Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1). No significant cross-reactivity or interference between Kazal Type Serine Protease Inhibitor Domain Containing Protein 1 (KAZALD1) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UL16 Binding Brotein 2 (ULBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UL16 Binding Brotein 2 (ULBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UL16 Binding Brotein 2 (ULBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UL16 Binding Brotein 2 (ULBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人Kazal Type Serine Protease Inhibitor Domain Containing Protein1(KAZALD1)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
