This assay has high sensitivity and excellent specificity for detection of Interleukin 4 (IL4). No significant cross-reactivity or interference between Interleukin 4 (IL4) and analogues was observed.
产品描述
The microplate provided in this kit has been pre-coated with an antibody specific to F1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to F2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F1+2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠白细胞介素4(IL4)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
