This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 5 (IGFBP5). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 5 (IGFBP5) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin (APLN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin (APLN) and unlabeled Apelin (APLN) (Standards or samples) with the pre-coated antibody specific to Apelin (APLN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin (APLN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin (APLN) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人胰岛素样生长因子结合蛋白5(IGFBP5)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
