This assay has high sensitivity and excellent specificity for detection of Hyaluronoglucosaminidase 1 (HYAL1). No significant cross-reactivity or interference between Hyaluronoglucosaminidase 1 (HYAL1) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Related Protein 14kDa (TRP14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin Related Protein 14kDa (TRP14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Related Protein 14kDa (TRP14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin Related Protein 14kDa (TRP14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠透明质酸氨基葡萄糖苷酶1(HYAL1)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
