This assay has high sensitivity and excellent specificity for detection of Four And A Half LIM Domains Protein 1 (FHL1). No significant cross-reactivity or interference between Four And A Half LIM Domains Protein 1 (FHL1) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxidized Glutathione (GSSG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxidized Glutathione (GSSG) and unlabeled Oxidized Glutathione (GSSG) (Standards or samples) with the pre-coated antibody specific to Oxidized Glutathione (GSSG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxidized Glutathione (GSSG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxidized Glutathione (GSSG) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人四个半LIM结构域蛋白1(FHL1)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
