This assay has high sensitivity and excellent specificity for detection of Serine/threonine-protein kinase mTOR (mTOR). No significant cross-reactivity or interference between Serine/threonine-protein kinase mTOR (mTOR) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SMAD family member 5 (SMAD5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SMAD family member 5 (SMAD5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SMAD family member 5 (SMAD5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SMAD family member 5 (SMAD5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人丝氨酸/苏氨酸蛋白激酶mTOR(mTOR)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
