This assay has high sensitivity and excellent specificity for detection of Factor Related Apoptosis (FAS). No significant cross-reactivity or interference between Factor Related Apoptosis (FAS) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hydroxyproline (Hyp) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hydroxyproline (Hyp) and unlabeled Hydroxyproline (Hyp) (Standards or samples) with the pre-coated antibody specific to Hydroxyproline (Hyp). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hydroxyproline (Hyp) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hydroxyproline (Hyp) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠因子相关凋亡(FAS)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
