This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF). No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.
产品描述
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Folic Acid (FA). A competitive inhibition reaction is launched between biotin labeled Folic Acid (FA) and unlabeled Folic Acid (FA) (Standards or samples) with the pre-coated antibody specific to Folic Acid (FA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Folic Acid (FA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Folic Acid (FA) level in the sample or standard.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠嗜酸性粒细胞趋化因子(ECF)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.