This assay has high sensitivity and excellent specificity for detection of Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A). No significant cross-reactivity or interference between Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A (DYRK1A) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biotinidase (BTD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biotinidase (BTD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biotinidase (BTD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biotinidase (BTD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该小鼠双特异性酪氨酸磷酸化调节激酶1A(DYRK1A)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
