This assay has high sensitivity and excellent specificity for detection of Chromodomain Helicase DNA Binding Protein 4 (CHD4). No significant cross-reactivity or interference between Chromodomain Helicase DNA Binding Protein 4 (CHD4) and analogues was observed.
产品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tryptophan (Trp) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tryptophan (Trp) and unlabeled Tryptophan (Trp) (Standards or samples) with the pre-coated antibody specific to Tryptophan (Trp). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tryptophan (Trp) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tryptophan (Trp) in the sample.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该小鼠色域解旋酶DNA结合蛋白4(CHD4)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
