This assay has high sensitivity and excellent specificity for detection of Betaine Homocysteine Methyltransferase (BHMT). No significant cross-reactivity or interference between Betaine Homocysteine Methyltransferase (BHMT) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人甜菜碱同型半胱氨酸甲基转移酶(BHMT)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
