This assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type VI (PADI6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type VI (PADI6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type VI (PADI6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type VI (PADI6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该小鼠17-β-羟基类固醇脱氢酶10型(HSD17b10)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.