This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat α-CTx. During the reaction, Rat α-CTx in the sample or standard competes with a fixed amount of Rat α-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat α-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat α-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.
产品特点
Colorimetric
保存建议
Biomatik品牌厂家建议该大鼠α-CTx(AlphaCrosslaps)ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
