This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. During the reaction, Rat PGF2α in the sample or standard competes with a fixed amount of Rat PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
产品特点
Colorimetric
保存建议
Biomatik品牌厂家建议该大鼠PGF2α（前列腺素F2Alpha）ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
