This ELISA Kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human αMSH. During the reaction, Human αMSH in the sample or standard competes with a fixed amount of Human αMSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human αMSH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human αMSH in the samples is then determined by comparing the OD of the samples to the standard curve.
产品特点
Colorimetric
保存建议
Biomatik品牌厂家建议该人αMSH（α-黑色素细胞刺激素）ELISA试剂盒产品，Short term: 4°C; Long term: see manual.
