The Phosphatidylethanol (PEth) ELISA detects PEth an alcohol biomarker that has been extracted from dried blood spot (DBS) cards. 50 µL of whole blood is required for duplicate data points. Detects PEth from whole blood spotted on a DBS (dried bloodspot) card. Utilizes known PEth containing samples analyzed by mass spectrometry as the standard curve for semi-quantitative measurement of PEth.The Phosphatidylethanol (PEth) ELISA assay is a competitive immunoassay. PEth in a sample extract competes with PEth on a plate for binding to a PEth specific HRP-conjugated antibody. Therefore the assay signal is inversely proportional to the amount of PEth present in the sample. This assay is designed for dried blood spot card samples and uses 4 small 3.2 mm (1/8 inch) punches for running duplicate or triplicate measurements. All major PEth molecular species are recognized by the antibody. The assay provides researchers a quick and robust method to screen for PEth in whole blood spotted DBS cards.PEth is a direct biomarker of alcohol ingestion. It is formed in the blood only when ethanol has been consumed. It is specific and can report previous alcohol consumption beginning a few hours after a drinking event and continuing 2-3 weeks later. Sensitive detection by mass spectrometry using whole blood or dried blood spot (DBS) cards is becoming generally accepted as the gold-standard for detecting past alcohol consumption. The Echelon PEth ELISA is the first commercially available immunoassay for detection of PEth in blood.
