二溴丙烷是一种巯基反应性蛋白质交联剂,当两个烷基化基团都与蛋白质反应时,会产生荧光产物。二溴丙烷具有390/450 nm的激发/发射光谱。 K. Ue等人(1985)。“用荧光交联剂稳定肌球蛋白亚片段1中的初级环。“Proceedings of the National Academy of Sciences 82(6):1658-1662.2)Konno K. K. Ue等人(2000年)。“在肌球蛋白S1中放置分子内交联的后果。“Proceedings of the National Academy of Sciences 97(4):1461-1466.3)Chen Z. B。L. Akin等人(2006年)。“Phospholamban的C-末端残基与心脏肌浆网的Ca 2+泵的交联,以探索跨膜结构域内的空间和功能相互作用。“Journal of Biological Chemistry 281(20):14163-14172.4)Lindzen M. K.- E. Gottschalk等人(2006年)。“FXYD蛋白质和Na+ K+-ATP酶之间的结构相互作用:Δ ±/Δ ²/FXYD亚单位化学计量和交联。“Journal of Biological Chemistry 281(9):5947- 5955.技术数据表
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Dibromobimane is a thiol reactive protein cross linking reagent that generates a fluorescent product when both of the alkylating groups have reacted with proteins. Dibromobimane has an excitation/ emission spectra of 390/450 nm.References1) Mornet D. K. Ue et al. (1985). "Stabilization of a primary loop in myosin subfragment 1 with a fluorescent crosslinker." Proceedings of the National Academy of Sciences 82(6): 1658-1662.2) Konno K. K. Ue et al. (2000). "Consequences of placing an intramolecular crosslink in myosin S1." Proceedings of the National Academy of Sciences 97(4): 1461-1466.3) Chen Z. B. L. Akin et al. (2006). "Cross-linking of C-terminal Residues of Phospholamban to the Ca2+ Pump of Cardiac Sarcoplasmic Reticulum to Probe Spatial and Functional Interactions within the Transmembrane Domain." Journal of Biological Chemistry 281(20): 14163-14172.4) Lindzen M. K.-E. Gottschalk et al. (2006). "Structural Interactions between FXYD Proteins and Na+ K+-ATPase: α/β/FXYD SUBUNIT STOICHIOMETRY AND CROSS-LINKING." Journal of Biological Chemistry 281(9): 5947-5955.Technical Data Sheet