这种新型的硫化氢（H2S）7-叠氮基-4-甲基香豆素（AzMC）选择性探针可用于监测胱硫醚B-合酶（CBS）催化的反应，该反应在人类硫代谢中起关键作用。失活的CBS的特征是血浆高半胱氨酸和H2S水平高，导致许多疾病状态的临床表现。AzMC用作荧光探针和同时的CBS活性可用于选择用于激活或抑制该酶的化合物，并且适合于高通量筛选和潜在治疗剂的开发。此外，AzMC已被用作人酚磺基转移酶（SULT 1A-1或P-PST-1）的底物结合位点的光亲和探针。M.K.索尔森T. Majtan J. P. Kraus A.M. Barrios“使用硫化氢选择性探针鉴定胱硫醚合酶抑制剂。“Angewandte Chemie（Int. ed. Eng.）2013年52 4641-4144。DOI：10.1002/anie.2013008412。G. Chen等人，“人类苯酚磺基转移酶（SULT 1A 1）底物结合位点的光亲和标记探针：7-叠氮基-4-甲基香豆素。蛋白质科学 1999 8 2151-2157 doi：10.1110/ps.8.10.21513. Q-L Xie等.“An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells”Analytical Methods 2017 DOI：10.1039/C7 AY 00862 G批量折扣可用，请发送电子邮件至echelon@echelon-inc.com获取信息。
产品描述
This novel and selective probe for hydrogen sulfide (H2S) 7-azido-4-methylcoumarin (AzMC) is useful for monitoring cystathionine B-synthase (CBS) catalyzed reactions which play a critical role in human sulfur metabolism. Deactivated CBS is characterized by high plasma levels of homocysteine and H2S resulting in clinical manifestations of numerous disease states. AzMC used as a fluorgenic probe and concurrent CBS activity can be used to select compounds for activation or inhibition of this enzyme and is suitable for high-throughput screening and the development of potential therapeutics. In addition AzMC has been used as a photoaffinitry probe for the substrate binding site of human phenol sulfotransferase (SULT1A-1 or P-PST-1).Featured in References1. M.K. Thorson T. Majtan J.P. Kraus A.M. Barrios "Identification of Cystathionine β-Synthase Inhibitors Using a Hydrogen Sulfide Selective Probe." Angewandte Chemie (Int. ed. Eng.) 2013 52 4641-4144. DOI: 10.1002/anie.2013008412. G. Chen et al. "Photoaffinity labeling probe for the substrate binding site of human phenol sulfotransferase (SULT1A1): 7-azido-4-methylcoumarin." Protein Sci. 1999 8 2151-2157 doi:10.1110/ps.8.10.21513. Q-L Xie et a. "An azidocoumarin-based fluorescent probe for imaging lysosomal hydrogen sulfide in living cells" Analytical Methods 2017 DOI: 10.1039/C7AY00862G Bulk discounts available please email echelon@echelon-inc.com for information.
