- 中文名称
Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay ELISA Kit (ENA)
- 英文名字
- Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay ELISA Kit (ENA)
- 供应商
- USBiological
- 产品货号
- E9012
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The United States Biological Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay ELISA Kit is intended for the detection of antibodies to Sm, RNP, Ro, La, Scl-70, and Jo-1 in human sera. The assay is to be used to detect antibodies in a single serum specimen. The results of the assay are to be used as an aid to the research of systemic lupus erythem atosis, mixed connective tissue disease, Sjogren syndrome, scleroderma, polymyositis, and dermatomyositis. This assay does not differerentiate these conditions.Systematic autoimmune disease is characterized by the presence of circulating autoantibodies directed to a wide variety of cellular antigens. Systemic lupus erythematosis (SLE), commonly referred to as Lupus, is the best known of these diseases. Other possible connective tissue diseases include mixed connective tissue disease (MCTD), Sjogren syndrome, sclerodema, and polymyositis/dermatomyositis. The Sm (Smith) antigen is composed of nuclear RNA and several polypeptides. Antibodies to Sm are present in approximately 30% of patients with SLE. Sm is a very specific marker for SLE. Sm antibodies are very rare in other autoimmune diseases and normals.RNP antigen is composed of RNA and several protein components. The antigen is closely associated with Sm antigen. Approximately 45% of patients with SLE have antibodies to RNP. RNP antibodies are also found in patients with discoid lupus, Sjogren syndrome, scleroderma, rheumatoid arthritis and MCTD. Very high titers to RNP is a characteristic of MCTD.Ro (SS-A) antigen consists of protein complexed with RNA. Approximately 60% of patients with Sjogren syndrome and 35% of SLE patients have antibodies to Ro. SLE patients with Ro antibodies alone (negative La/SSB) have a greater chance of developing nephritis.La (SS-B) antigen consists of a protein complexed to RNAs. Approximately 60% of patients with Sjogren syndrome and 15% of SLE patients have antibody to La. Ro (SSA) antibody occurs in virtually all sera with La antibody. However, some SLE patients have Ro only antibody profiles.Scl-70 antigen is a nuclear enzyme topoisomerase 1. Approximately 20-30% of patients with scleroderma have antibodies to Scl-70. The antibody is rarely seen in other conditions. Scl-70 antibodies are usually present in the diffuse subtype of scleroderma while antibodies to centromere proteins are dominant in the limited subtype of scleroderma or CREST syndrome.Jo-1 antigen is the cellular enzyme histidyl-tRNA synthetase. Approximately 30% of patients with polymyositis (PM) and 10% of patients with dermatomyositis (DM) have antibodies to Jo-1. Antibodies to Jo-1 are seen rarely in normals and other diseases except for those which overlap with PM-DM.Classically, antibodies to autoimmune antigens are detected by double immunodiffusion. However, the test is lengthy and suffers weak sensitivity. ELISAs combine greater sensitivity with ease of use. Many ELISAs have been developed and validated for detecting autoantibodies to various antigens.Kit Components:1. Sm, RNP, Ro, La, Scl-70, Jo-1 antigen coated microassay plate 1x96 wells2. Serum Diluent, 1x30ml. Supplied as a liquid in buffer, BSA, Tween-20, 0.1% proclin. 3. Positive Control, High, 1x400ul. Supplied as a liquid in < 0.1%, sodium azide, 0.01% pen/strep.4. Calibrator, 1x400ul. Supplied as a liquid in in < 0.1%, sodium azide, 0.01% pen/strep. 5. Negative Control, 1x400ul. Supplied as a liquid in < 0.1%, sodium azide, 0.01% pen/strep.6. Positive Control, Low, 1x400ul. Supplied as a liquid in < 0.1%, sodium azide, 0.01% pen/strep.7. IgG, IgA, IgM (HRP) Goat x human, 1x15ml. Supplied as a liquid in 0.1% proclin, gentamicin.8. Wash Buffer, 20X, 1x50ml. Dilute 1 part concentrate + 19 parts ddH2O. Supplied as a liquid in TBS, Tween-80, 0.1% proclin.9. TMB (Tetramethylbenzidine), 1x15ml. The reagent should remain closed when not in use. If allowed to evaporate, a precipitate may form in the reagent wells. 10. Stop Solution, 1x15ml. Supplied as a liquid 1N H2SO4 solution. Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay ELISA Kit (ENA)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Extractable Nuclear Antigen, Human, IgG, IgA, IgM BioAssay ELISA Kit (ENA)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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