- 中文名称
Ethanol Assay Kit, BioAssay, LHigh Sensitivity
- 英文名字
- Ethanol Assay Kit, BioAssay, LHigh Sensitivity
- 供应商
- USBiological
- 产品货号
- E8476-55
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) has Applications in basic research, drug discovery, clinic studies and in the alcoholic industry.Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. Ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sample.Applications:Direct Assays: ethanol in serum, plasma, urine and saliva samples.Pharmacology: effects of drugs on alcohol metabolism.Key Features:Sensitive and accurate. Detection limit 0.0008 vol % (140uM or 8ppm), linearity up to 0.1% ethanol in 96-well plate assay.Convenient. The procedure involves adding working reagent, incubating for 30 min and stropping reaction. No 37°C heater is needed.High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.Kit Contents: (100 tests in 96-well plates)Assay Buffer: 10ml NAD Solution: 1mlMTT Solution: 1.5ml Enzyme Mix: 120ulStop Reagent: 12ml Standard: 1.5ml 1% ethanolStorage conditions. Store Stop Reagent at room temperature and all other reagents at -20°C. Shelf life: 6 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:1. Calibration Curve. Prepare 0.1% alcohol Premix by mixing 25ul 1% Standard and 225ul distilled water. Dilute standard as follows.Transfer 10ul standards into wells of a clear bottom 96-well plate.No Premix+H2O Vol (ul) Ethanol (%)1 100ul+0ul 100 0.102 60ul+40ul 100 0.063 30ul+70ul 100 0.034 0ul+100ul 100 0Samples: add 10ul sample per well in separate wells.IMPORTANT: saliva samples should be diluted 10-fold in PBS prior to assay.2. Reaction. Spin the Enzyme Mix tube briefly before pipetting. For each well of reaction, prepare Working Reagent by mixing 80ul Assay Buffer, 1ul Enzyme Mix, 2.5ul NAD and 14ul MTT. Fresh reconstitution is recommended. Add 90ul Working Reagent per well quickly. Tap plate to mix briefly and thoroughly. Incubate 30 min at room temperature. Add 100ul Stop Reagent per well. Tap plate to mix.3. Read optical density at 565 nm (520-600nm).4. Calculation:. Subtract blank (water, #4) from OD values for the standard wells. Plot Standard Curve (DOD vs Standard ethanol concentrations) to determine the slope. Sample ethanol concentration is calculated,[Ethanol] =ODSAMPLE–ODBLANKSlope× n (%)where ODSAMPLE and ODBLANK are the OD565nm values of the sample and blank (water, #4). n is the dilution factor (n=10 for saliva). Note: if the sample ethanol concentrations are higher than 0.1%, dilute sample in distill water and repeat this assay. Multiply the results by the dilution factor.Conversions: 1 vol % ethanol equals 170mM or 785 mg/dL.Materials Required, But Not Provided:Pipeting (multi-channel) devices. Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.General Considerations:1. This assay is based on an enzyme-catalyzed kinetic reaction. Addition of Working Reagent should be quick and mixing should be brief but thorough. Use of multi-channel pipettor is recommended.2. The following substances interfere and should be avoided in sample preparation: ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%).
- 产品特点
- 针对Ethanol Assay Kit, BioAssay, LHigh Sensitivity该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Ethanol Assay Kit, BioAssay, LHigh Sensitivity产品后将其置于RT/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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