- 中文名称
Ethanol Assay Kit, BioAssay
- 英文名字
- Ethanol Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- E8476-54
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) finds applications in basic research, drug discovery, clinic studies and winery.Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. Ethanol assay kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.Applications:Ethanol determination in alcohol containing samples such as beverages (e.g. wine, beer) and yeast cultures. For samples containing less than 0.1% alcohol such as serum or plasma, Specific Ethanol Assay Kit is recommended.Key Features:Sensitive and accurate. Detection range 0.04–4% alcohol in 96-well plate assay.Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a highthroughput 96-well plate assay for thousands of samples per day.Versatility. Assays can be executed in 96-well plate or cuvet.Kit Contents: (500 tests in 96-well plates)Reagent A: 50ml Reagent B: 50ml10% TCA: 50ml Standard: 2ml 10% (v/v) ethanolStorage conditions. The kit is shipped at room temperature. Store reagents at room temperature and the ethanol standard at 4°C. Shelf life: 12 months after receipt.Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Procedure using 96-well plate:1. Prepare 600ul 2% Premix by mixing 120ul 10% Standard and 480ul distilled water. Dilute standard as follows. Transfer 100ul standards and samples into wells of a clear bottom 96-well plate.No Premix+H2O Vol (ul) Ethanol (%)1 150ul+0ul 150 2.002 120ul+30ul 150 1.603 90ul+60ul 150 1.204 60ul+90ul 150 0.805 45ul+105ul 150 0.606 30ul+120ul 150 0.407 15ul+135ul 150 0.208 0ul+150ul 150 02. Add 100ul Reagent A quickly using a multi-channel pipettor. Tap plate lightly to mix.3. Incubate 8 to 30 min at room temperature. The reagent color changes from yellow to visibly bluish in wells 1-4. Add 100ul Stop Reagent B quickly using a multi-channel pipettor. Tap plate to mix.4. Read OD at 570-600nm (peak 580nm).Procedure using cuvette:1. Prepare 2%, 1%, 0.5% standards and use distilled water as blank control. Transfer 400ul diluted Standards and 400ul samples to 1.5-mL centrifuge tubes.2. Add 400ul Reagent A quickly to each tube and vortex briefly to mix.3. Incubate 8 to 30 min at room temperature. Add 400ul Reagent B quickly and mix briefly.4. Transfer to cuvettes and read OD at 570-600nm (peak 580nm).Note: for the cuvette assay, it is recommended that an interval be applied between additions, e.g., add Reagent A to Tube 1 and 1 min later to Tube 2 etc. After the incubation step is completed, add the Stop Reagent B to Tube 1 and 1 min later to Tube 2 etc. This will ensure identical incubation time between tubes.Calculation:Subtract blank OD (water, #8) from the standard OD values and plot the OD against standard alcohol concentrations. Determine sample ethanol concentration from the standard curve.Conversions: 1% (v/v) ethanol equals 170mM or 785mg/dL.Materials Required, But Not Provided:Pipeting (multi-channel) devices.Procedure using 96-well plate:Clear-bottom 96-well plates (e.g. Corning Costar) and plate reader.Procedure using cuvette:Centrifuge tubes, table centrifuge, cuvets and spectrophotometer.General Considerations:1. If sample contains glucose or glycerol, use Specific ethanol assay kit. For samples containing only sugars (e.g. glucose), use Saccharide Removal Kit to remove the interferents prior to assay with the Ethanol Kit.2. This assay is based on a kinetic reaction. Addition of Reagent A and B (Stop reagent) should be quick and mixing should be brief but thorough.3. Sample pretreatment. Proteinaceous samples, e.g. plasma, serum, culture media, should be deproteinated by adding 1 vol sample to 2 vol 10% TCA (provided). Pellet for 5 min at 14,000 rpm on a table centrifuge, carefully transfer supernatant for assay (n=3). Saliva and urine can be analyzed directly (n=1). For wines, dilute samples to approximately 1 to 2% prior to assay.
- 产品特点
- 针对Ethanol Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Ethanol Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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