BioVision’s Mouse Carcinoembryonic Antigen (CEA) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CEA. This assay employs an antibody specific for mouse CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
产品特点
Detection Range: 62.50-4000 pg/mL, Sensitivity: 37.50 pg/mL, Precision: Coefficient of variation is < 10%, Specificity: No Significant cross-reactivity or interference between Mouse CEA and analogues was observed.