se in tissue remodeling. BioVision’s MMP-2 ELISA Kit is based on Sandwich ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human MMP-2. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human MMP-2. The concentration of Human MMP-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.
产品特点
Coefficient of variation is < 10%., Specificity: This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed., Detection Range: 0.78—50 ng/ml, Sensitivity: 0.47 ng/ml
