Pyruvate kinase is the enzyme that catalyzes the final step of glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP), yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase is present in four distinct, tissue-specific isozymes, each consisting of particular kinetic properties necessary to accommodate the variations in metabolic requirements of diverse tissues. BioVision’s PK ELISA kit is a sandwich ELISA assay for the quantitative measurement of PK in human serum, plasma and cell culture supernatants in 90 minutes. The density of color is proportional to the amount of PK captured from the samples.
产品特点
Easy, convenient and time-saving method to measure the level of PK in human Serum, plasma, urine, cell culture samples, biological fluid.
Detection Range: 8 - 500 pg/ml
Sensitivity: < 2.2 pg/ml
