Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. BioVision’s CCND1 ELISA kit is a sandwich ELISA assay for the quantitative measurement of human CCND1 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human CCND1 captured from the samples.
产品特点
Easy, convenient and time-saving method to measure the level of CCND1 alpha in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.313-20 ng/ml
Sensitivity: < 0.188 ng/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
