- 中文名称
Endothelin-1, BioAssay ELISA Kit (Human)
- 英文名字
- Endothelin-1, BioAssay ELISA Kit (Human)
- 供应商
- USBiological
- 产品货号
- E3650-03
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Endothelin-1, Human BioAssay ELISA Kit is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.Principle of the Test:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for ET-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ET-1 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for ET-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ET-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.Kit Components:E3650-03A: Microtiter Strips, 1x96 wellsE3650-03B: Endothelin-1 Mab (HRP), 1x21mlE3650-03C: Standard, 1x1vialE3650-03D: Assay Diluent, 1x18mlE3650-03E: Calibrator Diluent, 1x21mlE3650-03F: Wash Buffer, 25X, 1x21mlE3650-03G: Color Reagent A, 1x12mlE3650-03H: Color Reagent B, 1x12mlE3650-03J: Stop Solution, 2N H2SO4, 1x6mlSpecificity: This assay recognizes natural and synthetic Endothelin-1.The factors listed below were prepared at 250pg/ml in Calibrator Diluent and assayed for crossreactivity. Preparations of the following factors at 250pg/ml in a low level control were assayed for interference. No significant crossreactivity or interference was observed.Human peptides:Big Endothelin-1 (aa16-38), Big Endothelin-2, Endothelin-2, Sarafotoxin S6b, Sarafotoxin S6cRat peptides:Big Endothelin-39Bovine peptides:Big Endothelin-39Human Endothelin-2 shows 23.4% crossreactivity in this assay.Human/Rat Endothelin-3 shows 0.5% crossreactivity in this assay.Sensitivity: Thirty-four assays were evaluated and the minimum detectable dose (MDD) of Endothelin-1 ranged from 0.031-0.207pg/ml. The mean MDD was 0.087pg/ml.The MDD was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.Precision:Intra-assay Precision:Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision.Mean (pg/ml): 3.00, 7.34, 14.7Standard Deviation: 0.120, 0.170, 0.280CV (%): 4.0, 2.3, 1.9Inter-assay Precision:Three samples of known concentration were tested in 40 separate assays to assess inter-assay precision.Mean (pg/ml): 3.05, 7.43, 14.4Standard Deviation: 0.231, 0.438, 0.759CV (%): 7.6, 5.9, 5.3Storage and Stability:Store kit components at 4°C. Stable for 6 month after receipt at 4°C. After opening/reconstitution of reagents, stable for 1 month. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Endothelin-1, BioAssay ELISA Kit (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Endothelin-1, BioAssay ELISA Kit (Human)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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