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细胞分析试剂盒
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中文名称

Estriol, Human, BioAssay ELISA Kit

英文名字
Estriol, Human, BioAssay ELISA Kit
供应商
USBiological
产品货号
E3560-11
产品报价
¥1/1Kit
产品说明书
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背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
The United States Biological Estriol, Human, BioAssay ELISA Kit is a competitive immunoenzymatic colorimetric method for quantitative determination of Free Estriol concentration in serum and plasma.Estriol (also oestriol) is one of the three main estrogens produced by the human body. It is only produced in significant amounts during pregnancy as it is made by the fetus. During pregnancy the production of estriol depends on an intact maternal-placental-fetal unit. Fetal-placental production of estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than non-pregnant levels. In the maternal circulation, estriol undergoes rapid conjugation in the liver followed by urinary excretion with a half-life of ~20 minutes. Since normal estriol production depends on an intact maternal-placental-fetal circulation and functional fetal metabolism, maternal estriol levels have been used to monitor fetal status during pregnancy, particularly during the third trimester. DHEA is produced by the adrenal cortex of the fetus, this is converted to estriol by the placenta. If levels are abnormally low in a pregnant woman, this may indicate a problem with the development in the child. Levels of estriol in non-pregnant women do not change much after menopause, and levels are not significantly different from levels in men.Free Estriol (antigen) in the sample competes with horseradish-peroxidase Estriol (enzyme-labeled-antigen) for binding onto the limited number of anti Estriol (antibody) sites on the microplates (solid phase). After incubation, the bound/free separation is performed by a simple solid-phase washing. The enzyme substrate (H2O2) and the TMB-Sustrate (TMB) are added. After an appropriate time has elapsed for maximum colour development, the enzyme reaction is stopped and the absorbance are determined. Free Estriol concentration in the sample is calculated based on a series of standard. The colour intensity is inversely proportional to the Free Estriol concentration in the sample. Kit Components: 1. Free Estriol Standards: 4x1ml2. Incubation buffer: 1X30ml 3. Estriol (HRP): 1X400ul 4. Microtiter Plate: 1x96 wells 5. Tetramethylbenzidine (TMB): 1x12ml6. Stop Solution: 1X12ml (1N HCl) Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Estriol, Human, BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Estriol, Human, BioAssay ELISA Kit产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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