- 中文名称
Escherichia coli, O157, BioAssay ELISA Kit
- 英文名字
- Escherichia coli, O157, BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- E3500-03
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Strains of Escherichia coli that produce Shiga-like toxins (SLTs), also known as Verocytotoxins (VTs), are an important cause of human disease. Clinical manifestations of infection include diarrhea, hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). Although Shiga-like toxin producing E. coli (SLTEC) belong to many O serogroups, serotypes O157/H7 and O157/H are the predominant SLTEC associated with HC and HUS.Outbreaks of HC and HUS due to E. coli O157 have been linked to consumption of undercooked ground beef (Riley et al, 1983; Wells et al 1983; Padhye and Doyle, 1992). Prevalence studies indicate that up to 3.7% of retail meats may contain E. coli O157 (Doyle and Schoeni 1987). Although this may seem low compared to some other foodborne pathogens, the seriousness of disease caused by E. coli O157 has made this pathogen a major food safety concern.Detection of E. coli O157/H7 in meats by cultural methods is time consuming, requiring several days to obtain presumptive positive results. Typically, meat samples are cultured in primary enrichment broth which is plated onto sorbitol-MacConkey (SMAC) agar. Non-sorbitol fermenting colonies are then subcultured for identification by serotyping or related methods. As some E. coli O157 strains associated with HC and HUS ferment sorbitol (Karch et al 1993), this approach may give false negative results.The United States Biological E. coli O157 ELISA is a rapid and reliable test which significantly reduces the time required to screen foods for the presence of E. coli O157. Primary enrichment cultures grown for 8-16 hours can be tested in less than one hour, allowing ELISA-negative product to be released within 24 hours. Enrichment broths presumptively positive for E. coli O157 on the basis of positive ELISA tests can be cultured further for confirmation by standard methods.Intended Use:The United States Biological E. coli O157 assay is an enzyme-linked immunosorbent assay (ELISA) that may be used to screen food products for the presence of E. coli O157 antigen.Test Principle:The United States Biological E. coli O157 ELISA is a double antibody (sandwich) ELISA utilizing specific anti-E. coli O157 antibodies coated to microwells. After addition of the sample and the enzyme conjugate, a positive reaction (indicating the presence of E. coli O157 antigen) produces a deep blue color. Addition of the Stop Solution ends the assay and turns the blue color to yellow. The results may be read visually or with an ELISA reader.Kit Components:1. Microtiter plate 1x96 wells each.2. Test strip holder: 1.3. Enzyme Conjugate: 1x11ml of peroxidase polyclonal antibody.4. Positive control: 1x1ml of killed cells in a buffered base.5. Negative control: 1x1ml of buffered base.6. Chromogen: 1x11ml of the chromogen tetramethylbenzidine (TMB).7. Wash Concentrate 20x: 2x25 ml of concentrated buffer and surfactant with preservative.8. Stop solution: 1x11ml of 1M phosphoric acid.Storage and Stability:Store components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Escherichia coli, O157, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Escherichia coli, O157, BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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