- 中文名称
Erythropoietin, Human (EPO), ELISA Kit, BioAssay
- 英文名字
- Erythropoietin, Human (EPO), ELISA Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- E3455-08H
- 产品报价
- ￥1/1Kit
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Intended Use:The EPO Human ELISA Kit is intended for the quantitative determination of Erythropoietin (EPO) in human serum. This assay is intended for in vitro diagnostic use, as an aid in the diagnosis of anemias and polycythemias. With the advent of the administration of recombinant erythropoietin as a biologic therapy to increase red blood cell mass, an erythropoietin assay may be used also to aid in the prediction and monitoring of response to recombinant erythropoietin treatment in persons with anemias.Introduction:Erythropoietin (EPO) is a heavily glycosylated protein with a molecular weight of about 30,000-34,000 Daltons. Human EPO is a polypeptide consisting of 165 amino acids, containing one O-linked and three N-linked carbohydrate chains. The recombinant EPO is a good substitute for the native protein for use in an immunoassay. Serum EPO levels are dependent on the rate of production and the rate of clearance of the protein. Ninety percent of EPO is produced in the peritubular cells of the adult kidney in response to a decrease in tissue oxygenation. There is evidence indicating that the protein on these cells which detects oxygen saturation of the blood is a heme-containing moiety. As the pO2 of the plasma, a function of the hematocrit decreases, EPO concentration will increase. There are also observations suggesting that normally there is an inverse correlation between serum EPO levels and red blood cell mass.Quantitation of serum erythropoietin concentration serves as a diagnostic adjunct in determining the cause of anemia or erythrocytosis. Aplastic anemia, hemolytic anemia and anemia due to iron deficiency all result in serum EPO elevation. Whereas, EPO levels in patients with secondary anemia due to renal failure and other disorders such as acquired immune deficiency syndrome (AIDS) are generally inappropriately low for the degree of anemia. This is mostly likely caused by an impaired ability of the diseased kidney to produce adequate quantities of EPO. Low concentrations of EPO may give an early warning of kidney transplant rejection. EPO also can be used to monitor AIDS patients undergoing Zidovudine (AZT) therapy. An increased concentration of EPO verifies that anemia associated with AZT therapy is due to red cell hypoplasia or apliasia.Polycythemia rubra vera, or primary erythrocytosis (an increase of red blood cell mass) results from unstimulated over production of erythrocytes. Hence, the increase in the hemoglobin causes decreased production of EPO, which results in subnormal levels of serum EPO9. Secondary polycythemias, which are also characterized by an increase in the total red blood cell mass, occur as a physiological response to elevated levels of circulatory EPO caused by tissue hypoxia. The hypoxia may be due to such factors as pulmonary fibrosis, cardiovascular disease, prolonged exposure to high altitude, abnormal forms of hemoglobin or drug treatment. Some tumors produce EPO and, in these cases, EPO may be used as a tumor marker to monitor the effectiveness of treatment.Kit Components:E3455-08H1: Microwell plate, 1x96 wells. Pre-coated with StreptavidinE3455-08H2: EPO (Biotin), 1x3.5ml. Suplied as a liquid with ProClin 300E3455-08H3: EPO (HRP), 1x3.5ml. Supplied as a liquidE3455-08H4: Calibrator, 0mIU/ml. 1x4ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H5: Calibrator, 10.8mIU/ml. 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H6: Calibrator, 26.8mIU/ml. 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H7: Calibrator, 54mIU/ml. 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H8: Calibrator, 167mIU/ml. 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H9: Calibrator, 498mIU/ml. 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H12: Control 1 (14.3-22.6mIU/ml), 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin HydrochlorideE3455-08H13: Control 2 (164-260mIU/ml), 1x2ml. Supplied as a lyophilized powder with Ciprofloxacin Hydrochloride *E3455-08H14: Wash Buffer (20x), 1x30ml. Supplied as a liquid with Ciprofloxacin HydrochlorideE3455-08H15: TMB Substrate, 1x20ml. Supplied as a liquidE3455-08H16: Stop Solution (1N H2SO4), 1x20ml. Supplied as a liquidStorage and Stability:Store *E3455-08H14 at RT. Store other components at 4°C. Store reconstituted calibrators and controls at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vials prior to removing the cap.
- 产品特点
- 针对Erythropoietin, Human (EPO), ELISA Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Erythropoietin, Human (EPO), ELISA Kit, BioAssay产品后将其置于RT/4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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